Plant Disease


Publications
259

Development of a TaqMan assay for the detection of ‘Candidatus Phytoplasma brasiliense’ and assessment by high resolution melt-curve analysis (HRMA)

Citation
Lane et al. (2024). Plant Disease
Names
Ca. Phytoplasma brasiliense
Abstract
‘Candidatus Phytoplasma brasiliense’ (CPB) is a phytoplasma originally discovered in South America and is known to infect a wide variety of economically important crops. It is most prevalent in Hibiscus spp. where it causes witches broom symptoms and papaya where it causes bunchy top. Recently, CPB was documented for the first time in North America in a new host, globe sedge. In this study two qPCR assays are developed, one utilizing high resolution melt curve analysis (HRMA) based on the secA

Momordica charantia Is a Novel Host of 'Candidatus Phytoplasma malaysianum'-Related Strains Associated with Bitter Melon Stem Fasciation Disease in China

Citation
Bottner-Parker et al. (2024). Plant Disease
Names
Ca. Phytoplasma malaysianum Ca. Phytoplasma luffae
Abstract
Momordica charantia, also known as bitter melon, bitter gourd, and bitter squash, is a member of the Cucurbitaceae family and is widely grown in tropical and subtropical regions for its edible fruit and medicinal properties (Alves et al. 2017). In April 2022, bitter melon plants exhibiting stem fasciation and excessive tendril symptoms were observed in a 50-acre vegetable farm in Yijia Village, Weishan Yizu Huizu Autonomous County, Dali, Yunnan Province, China (Fig. 1). The farm primarily grew

Diversity of ‘Candidatus Liberibacter asiaticus’ Strains in Texas Revealed by Prophage Sequence Analyses

Citation
De Leon et al. (2024). Plant Disease 108 (6)
Names
Ca. Liberibacter asiaticus
Abstract
Prophages/phages are important components of the genome of ‘Candidatus Liberibacter asiaticus’ (CLas), an unculturable alphaproteobacterium associated with citrus huanglongbing (HLB) disease. Phage variations have significant contributions to CLas strain diversity research, which provide critical information for HLB management. In this study, prophage variations among selected CLas strains from southern Texas were studied. The CLas strains were collected from three different CLas inhabitant env

Identification of ‘Candidatus Liberibacter asiaticus’, the Huanglongbing Bacterium, in Citrus from Colombia

Citation
Chaves-Sierra et al. (2024). Plant Disease 108 (5)
Names
Liberibacter Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter’ spp. are the most prevalent microorganisms in the citrus plant, associated with citrus huanglongbing, which are transmitted by psyllid vectors. In Colombia, the vector Diaphorina citri Kuwayama has been reported in different regions, but ‘Ca. Liberibacter asiaticus’ (CLas) has only been detected in insect vectors, not in citrus host plants. To identify the presence and quantify the pathogen in citrus tissues, we employed a combined strategy that involved three techniqu

Molecular Identification of ‘Candidatus Phytoplasma malaysianum’-Related Strains Associated with Areca catechu Palm Yellow Leaf Disease and Phylogenetic Diversity of the Phytoplasmas Within the 16SrXXXII Group

Citation
Yu et al. (2024). Plant Disease 108 (5)
Names
Ca. Phytoplasma malaysianum
Abstract
Areca catechu palm is an important cash plant in Hainan Island of China and also in the tropical regions of the world. A. catechu palm yellow leaf (AcYL) disease caused by phytoplasmas is a devastating disease for plant production. In the study, the phytoplasmas associated with the AcYL disease were identified and characterized based on their conserved genes, and genetic variation and phylogenetic relationship of the phytoplasma strains in the 16SrXXXII group were demonstrated. The results indi

An Inhibitor-Monitorable Single-Tube Duplex Quantitative Real-Time PCR Assay for the Detection of ‘Candidatus Liberibacter asiaticus’

Citation
Huang et al. (2024). Plant Disease 108 (5)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing (HLB) is a citrus infectious disease caused by ‘Candidatus Liberibacter’ spp. Recently, it has begun to spread rapidly worldwide, causing significant losses to the citrus industry. Early diagnosis of HLB relies on quantitative real-time PCR assays. However, the PCR inhibitors found in the nucleic acid extracted from plant materials pose challenges for PCR assays because they may result in false-negative results. Internal standard (IS) can be introduced to establish a single-tube d

First Report of ‘Candidatus Phytoplasma asteris’-related Strain (16SrI group) Associated with Small Leaves, Leaf Yellowing, and Shoot Proliferation of Morning Glory (Ipomoea biflora) in Taiwan

Citation
Hung et al. (2024). Plant Disease
Names
Ca. Phytoplasma asteris
Abstract
Ipomoea biflora L., commonly known as morning glory, is an herbaceous vine plant in the Convolvulaceae family and is widespread at low elevations in Taiwan and other East Asian countries. In September 2023, six I. biflora plants exhibiting small leaves, leaf yellowing, and shoot proliferation were observed in a vacant lot in Taiwan Agricultural Research Institute (TARI), Wufeng District, Taichung, Taiwan, representing 100% disease incidence in the area. All the symptomatic morning glory climbed