Publications

4467

ABSTRACTA real-time PCR assay using Taqman probes was developed to detect and quantifyMycoplasma haemofelisand “CandidatusMycoplasma haemominutum” in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.

ABSTRACT We report on a new Actinobaculum species, “ Actinobaculum massiliae ,” isolated from the urine of an elderly woman with recurrent cystitis. Its phenotypic pattern was similar to those of both of the other Actinobaculum species described to date. On 16S rRNA sequencing, the Marseille isolate shared 95% homology with Actinobaculum suis , 92 to 93% homology with Actinobaculum schaalii , 91 to 92% homology with Arcanobacterium spp., and 87 to 90% homology with Actinomyces species. A bootstrap value of 99% supports the node separating the Actinobaculum sp. from its closest neighbor ( A. suis ). In conclusion, on the basis of phenotypic, genotypic, and phylogenetic assessments, we show that the Marseille isolate is a previously unrecognized organism within the Actinobaculum genus, and we propose placement of the organism in the taxon “ Actinobaculum massiliae .”