Isolate A649(T) (=CBAS 716(T) = CBRVS P1061(T)) obtained from viscera of the healthy pufferfish Sphoeroides spengleri (Family Tetraodontidae, pufferfish). Their cells are Gram-negative, facultative aerobic, motile rods, and 0.5–1.0 μm in diameter. Colonies are circular with whole margin and papillary elevation on Marine Agar and TCBS (Green colony). A649(T) is positive for d-mannitol utilization and indole production based on an in silico phenotype.