BioSample Accession(s)

SAMEA6951078, ERS4660955

geographic location (depth)

0.5

assembly software

megahit

environment (material)

water

sulfate

0.74

ferric iron

8

collection date

2015-08-08

ferrous iron

1.6

sample derived from

ERS4600355

ammonium

0.498919008814236

geographic location (longitude)

25.11388888888889

metagenomic OTU

anoxic_mOTU_1187

binning parameters

min_len:1500,min_bin_size:10000,maxP:93,minS:50

contamination score

0.0

nitrate

2.2740138279394

gtdb_taxonomy

d__Bacteria;p__Proteobacteria;c__Alphaproteobacteria;o__Pelagibacterales;f__Pelagibacteraceae;g__Fonsibacter

water environmental package

water

sample owner

Leyden Fernandez

ENA-FIRST-PUBLIC

2020-06-15

ENA-LAST-UPDATE

2020-06-18

completeness software

checkm

temperature

19.8

collection data

metagenome

environment (biome)

lentic water body

oxygen

8.55

environment (feature)

lake

geographic location (country and/or sea)

Finland

ENA-CHECKLIST

ERC000047

binning software

metabat

organism

uncultured Alphaproteobacteria bacterium

assembly quality

Many fragments with little to no review of assembly other than reporting of standard assembly statistics

phosphate

0.021058971437717

taxonomic identity marker

gtdbtk followed by sourmash-lca, see publication

completeness score

99.9

dissolved carbon dioxide

0.036

geographic location (latitude)

61.20805555555556

scientific_name

uncultured Alphaproteobacteria bacterium

common name

investigation type

metagenome

metagenomic source

lake water metagenome

project name

microbes in stratified freshwaters

sequencing method

NovaSeq

methane

0.355

iron

9.6

region

Evo

isolation_source

lake water

site name

Alinen Mustajärvi

BioSample Accession(s)

SAMEA6872791, ERS4600355

geographic location (depth)

0.5

environment (material)

water

sulfate

0.74

ferric iron

8

collection date

2015-08-08

ferrous iron

1.6

ammonium

0.498919008814236

geographic location (longitude)

25.11388888888889

nitrate

2.2740138279394

water environmental package

water

sample owner

Leyden Fernandez

ENA-FIRST-PUBLIC

2020-06-15

ENA-LAST-UPDATE

2020-06-18

temperature

19.8

collection data

metagenome

environment (biome)

lentic water body

Lake_code

AM

oxygen

8.55

environment (feature)

lake

geographic location (country and/or sea)

Finland

ENA-CHECKLIST

ERC000024

organism

lake water metagenome

phosphate

0.021058971437717

Run

R1

dissolved carbon dioxide

0.036

geographic location (latitude)

61.20805555555556

scientific_name

lake water metagenome

common name

investigation type

metagenome

project name

microbes in stratified freshwaters

sequencing method

NovaSeq

methane

0.355

iron

9.6

region

Evo

site name

Alinen Mustajärvi

Metadata as EBI XML

<EXPERIMENT_SET>
<EXPERIMENT accession="ERX4154816" alias="webin-reads-microbes_in_stratified_freshwaters_-_lake_Alinen_Mustaj&#xE4;rvi_sample_AM1" center_name="Department of Aquatic Sciences and Assessment">
  <IDENTIFIERS>
    <PRIMARY_ID>ERX4154816</PRIMARY_ID>
    <SUBMITTER_ID namespace="Department of Aquatic Sciences and Assessment">webin-reads-microbes_in_stratified_freshwaters_-_lake_Alinen_Mustaj&#xE4;rvi_sample_AM1</SUBMITTER_ID>
  </IDENTIFIERS>
  <TITLE>Raw reads: microbes_in_stratified_freshwaters_-_lake_Alinen_Mustaj&#xE4;rvi_sample_AM1</TITLE>
  <STUDY_REF accession="ERP122124">
    <IDENTIFIERS>
      <PRIMARY_ID>ERP122124</PRIMARY_ID>
      <SECONDARY_ID>PRJEB38681</SECONDARY_ID>
    </IDENTIFIERS>
  </STUDY_REF>
  <DESIGN>
    <DESIGN_DESCRIPTION>unspecified</DESIGN_DESCRIPTION>
    <SAMPLE_DESCRIPTOR accession="ERS4600355">
      <IDENTIFIERS>
        <PRIMARY_ID>ERS4600355</PRIMARY_ID>
        <EXTERNAL_ID namespace="BioSample">SAMEA6872791</EXTERNAL_ID>
      </IDENTIFIERS>
    </SAMPLE_DESCRIPTOR>
    <LIBRARY_DESCRIPTOR>
      <LIBRARY_NAME>lake Alinen Mustaj&#xE4;rvi sample AM1</LIBRARY_NAME>
      <LIBRARY_STRATEGY>WGS</LIBRARY_STRATEGY>
      <LIBRARY_SOURCE>METAGENOMIC</LIBRARY_SOURCE>
      <LIBRARY_SELECTION>RANDOM</LIBRARY_SELECTION>
      <LIBRARY_LAYOUT>
        <PAIRED/>
      </LIBRARY_LAYOUT>
    </LIBRARY_DESCRIPTOR>
  </DESIGN>
  <PLATFORM>
    <ILLUMINA>
      <INSTRUMENT_MODEL>Illumina NovaSeq 6000</INSTRUMENT_MODEL>
    </ILLUMINA>
  </PLATFORM>
  <EXPERIMENT_LINKS>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SAMPLE</DB>
        <ID>ERS4600355</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMISSION</DB>
        <ID>ERA2667839</ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-FASTQ-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX4154816&result=read_run&fields=run_accession,fastq_ftp,fastq_md5,fastq_bytes]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
    <EXPERIMENT_LINK>
      <XREF_LINK>
        <DB>ENA-SUBMITTED-FILES</DB>
        <ID><![CDATA[https://www.ebi.ac.uk/ena/portal/api/filereport?accession=ERX4154816&result=read_run&fields=run_accession,submitted_ftp,submitted_md5,submitted_bytes,submitted_format]]></ID>
      </XREF_LINK>
    </EXPERIMENT_LINK>
  </EXPERIMENT_LINKS>
  <EXPERIMENT_ATTRIBUTES>
    <EXPERIMENT_ATTRIBUTE>
      <TAG>ENA-STATUS-ID</TAG>
      <VALUE>4</VALUE>
    </EXPERIMENT_ATTRIBUTE>
  </EXPERIMENT_ATTRIBUTES>
</EXPERIMENT>
</EXPERIMENT_SET>