Genome sc|0003579


Database

NCBI Assembly

Accession

GCA_964203055.1

Genomics

Accession
NCBI Assembly:GCA_964203055.1
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 94.0%
  • Contamination: 0.0%
  • Quality: 94.0
Ribosomal and transfer RNA genes
  • 0 16S rRNAs
  • 0 23S rRNAs
  • tRNAs for 18 amino acids
Sequencing depth
10.0 ×
Source
Other features
  • G+C Content: 29.4%
  • Coding Density: 95.14%
  • Codon Table: 11
  • N50: 585,694 bp
  • Contigs: 3
  • Largest Contig: 585,694 bp
  • Assembly Length: 937,461 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
This is a metagenome-assembled genome (MAG) that was assembled with FLYE v2.9.1-b1780 (settings: --nano-corr –meta –no-alt-contigs) from combined long- and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Contigs ≥3 kbp were used for hybrid binning (tetranucleotide frequencies and coverage data) using MaxBin-2.2.7, MetaBAT and MetaBAT2 and DAS Tool-1.1.3 to combine their predictions. Bins were manually curated and CheckM v1.1.3 was used to estimate bin completeness, contamination, and strain heterogeneity. Sequencing depth was not estimated and thus set to 10x. rRNA genes were predicted with barrnap v0.9, tRNA genes were predicted with tRNAscan v1.4. Although the fragmentation of this long-read MAG is low (it consists of only 3 contigs), and it is highly complete (94%), it does not contain any rRNA genes. 
Automated checks
Complete

Last modified 3 months ago

Sample Metadata

BioSample entries
Date
Collection Date: 2019-04-03 • 2019-03-03
Location
Geographic Location Latitude: 47° 17′ 60″ N • 47° 18′ 2.4″ N
Geographic Location Longitude: 8° 34′ E • 8° 34′ 44″ E
View map of sampling locations
Toponym
Geographic Location Country And Or Sea: Switzerland
Environment
Isolation Source: freshwater lake
Broad Scale Environmental Context: freshwater biome • freshwater lake
Local Environmental Context: freshwater lake biome • large lake
Environmental Medium: natural lake • fresh water
Other
Depth: 5
Package
Ena Checklist: ERC000047ERC000024
All retrieved samples

Metadata retrieved 3 months ago

Sequencing Experiments

PromethION sequencing
Illumina NovaSeq 6000 paired end sequencing


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