Genome sc|0003580


Database

NCBI Assembly

Accession

GCA_965235955.1

Genomics

Accession
NCBI Assembly:GCA_965235955.1
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 99.5%
  • Contamination: 0.5%
  • Quality: 97.0
Ribosomal and transfer RNA genes
  • 1 16S rRNA (up to 100.0%)
  • 1 23S rRNA (up to 100.0%)
  • tRNAs for 20 amino acids
Sequencing depth
28.3273 ×
Source
Other features
  • G+C Content: 29.4%
  • Coding Density: 93.71%
  • Codon Table: 11
  • N50: 946,070 bp
  • Contigs: 8
  • Largest Contig: 946,070 bp
  • Assembly Length: 1,246,959 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
This is a metagenome-assembled genome (MAG) that was assembled with FLYE v2.9.1-b1780 (settings: --nano-corr –meta –no-alt-contigs) from combined long- and short-read sequencing (Oxford Nanopore and Illumina NovaSeq). Contigs ≥3 kbp were used for hybrid binning (tetranucleotide frequencies and coverage data using cross-mapping of reads gained from the same lake, i.e., 12 samples in the case of Lake Malawi) using MaxBin-2.2.7, MetaBAT and MetaBAT2 and DAS Tool-1.1.3 to combine their predictions. Bins were manually curated and CheckM v1.1.3 was used to estimate bin completeness, contamination, and strain heterogeneity. rRNA genes were predicted with barrnap v0.9, tRNA genes were predicted with tRNAscan v1.4.
Automated checks
Complete

Last modified about 1 month ago

Sample Metadata

BioSample entries
Date
Collection Date: 2023-11-20 • 2023-11-23
Location
Geographic Location Latitude: 13° 55′ 55″ S • 13° 55′ 55″ S
Geographic Location Longitude: 34° 49′ 4.4″ E • 34° 49′ 4.4″ E
View map of sampling locations
Toponym
Geographic Location Country And Or Sea: Malawi
Environment
Isolation Source: Lake Malawi
Broad Scale Environmental Context: freshwater biome
Local Environmental Context: freshwater lake • freshwater lake biome
Environmental Medium: rift lake
Other
Ph: 8.74
Depth: 5
Temperature: 27.74
Chlorophyll: 0.04
Salinity: 0.12
Conductivity: 0.2555
Package
Ena Checklist: ERC000047ERC000024
All retrieved samples

Metadata retrieved about 1 month ago


© 2022-2025 The SeqCode Initiative
  All information contributed to the SeqCode Registry is released under the terms of the Creative Commons Attribution (CC BY) 4.0 license