This sample (TARA_B110000090) was collected at station TARA_173 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130708T0412Z_173_EVENT_PUMP) occurred at position latitudeN=78.9564 and longitudeE=79.4201, on date/time=2013-07-08T04:12:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000089.
- Further Details
- http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130708Z_173_WETLAB_BACT.pdf
- Nitrate Sensor
- 2.40637
- Environment (Feature)
- [SRF] surface water layer (ENVO:00010504)
- Temperature
- -0.083125
- Chlorophyll Sensor
- 0.00765
- Depth
- 5
- ENA-FIRST-PUBLIC
- 2019-10-15
- Sample Status
- This version can be used to provide data discovery services
- Size Fraction Lower Threshold
- 0.22
- ENA-LAST-UPDATE
- 2016-08-25
- Citation
- Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
- ENA-CHECKLIST
- ERC000030
- Sample Collection Device
- high volume peristaltic pump [HVP-PUMP]
- organism
- marine metagenome
- Latitude End
- 78.9222
- Event Label
- TARA_20130708T0412Z_173_EVENT_PUMP
- environment (feature) further details
- discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence
- scientific_name
- marine metagenome
- Latitude Start
- 78.9564
- Sampling Platform
- SV Tara
- Sampling Station
- TARA_173
- Longitude Start
- 79.4201
- Last Update Date
- 2016-04-23Z
- protocol description
- In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.
- Marine Region
- [AO] Arctic Ocean (MRGID:1906)
- Project Name
- Tara Oceans Expedition (2009-2013)
- Environment (Biome)
- Polar Biome
- Protocol Label
- SEQ-(100L)_W0.22-3
- Size Fraction Upper Threshold
- 3
- Environment (Material)
- water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
- Event Date/Time End
- 2013-07-08T07:55:00
- sample material label
- TARA_173_SRF_0.22-3
- Sampling Campaign
- TARA_20130629Z
- Oxygen Sensor
- 383.163125
- Longitude End
- 79.2246
- Salinity Sensor
- 33.595737
- Environmental Package
- water
- Event Date/Time Start
- 2013-07-08T04:12:00