This sample (TARA_B110000208) was collected at station TARA_178 during campaign TARA_20130629Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130715T0211Z_178_EVENT_PUMP) occurred at position latitudeN=77.1604 and longitudeE=73.2057, on date/time=2013-07-15T02:11:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000209.

Further Details

http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130715Z_178_WETLAB_BACT.pdf

Nitrate Sensor

99999

Environment (Feature)

[SRF] surface water layer (ENVO:00010504)

Temperature

1.243225

Chlorophyll Sensor

-0.0396

Depth

5

ENA-FIRST-PUBLIC

2019-10-15

Sample Status

This version can be used to provide data discovery services

Size Fraction Lower Threshold

0.22

ENA-LAST-UPDATE

2016-08-25

Citation

Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)

ENA-CHECKLIST

ERC000030

Sample Collection Device

high volume peristaltic pump [HVP-PUMP]

organism

marine metagenome

Latitude End

77.3025

Event Label

TARA_20130715T0211Z_178_EVENT_PUMP

environment (feature) further details

discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)

scientific_name

marine metagenome

Latitude Start

77.1604

Sampling Platform

SV Tara

Sampling Station

TARA_178

Longitude Start

73.2057

Last Update Date

2016-04-23Z

protocol description

In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.

Marine Region

[AO] Arctic Ocean (MRGID:1906)

Project Name

Tara Oceans Expedition (2009-2013)

Environment (Biome)

Polar Biome

Protocol Label

SEQ-(100L)_W0.22-3

Size Fraction Upper Threshold

3

Environment (Material)

water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)

Event Date/Time End

2013-07-15T06:04:00

sample material label

TARA_178_SRF_0.22-3

Sampling Campaign

TARA_20130629Z

Oxygen Sensor

348.536

Longitude End

73.2438

Salinity Sensor

34.056675

Environmental Package

water

Event Date/Time Start

2013-07-15T02:11:00

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