Genome sc|0004379


Database

NCBI Assembly

Accession

GCA_054906415.1

Genomics

Accession
NCBI Assembly:GCA_054906415.1
Type
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 99.85%
  • Contamination: 0.62%
  • Quality: 96.75
Ribosomal and transfer RNA genes
  • 1 16S rRNA (up to 100.0%)
  • 2 23S rRNAs (up to 41.0%)
  • tRNAs for 19 amino acids
Sequencing depth
307.0 ×
Source
Other features
  • G+C Content: 30.7%
  • Coding Density: 89.45%
  • Codon Table: 11
  • N50: 233,741 bp
  • Contigs: 13
  • Largest Contig: 699,134 bp
  • Assembly Length: 1,685,034 bp
  • Ambiguous Assembly Fraction: 0.3%
Submitter comments
The tRNA genes were identified using the Prokka pipeline while rRNA genes were detected with Barrnap. Although Barrnap indicated low completeness of the 16S rRNA gene in the genome assembly (presence of V1-V3 16S rRNA gene sequence only), full-length 16S rRNA gene sequencing using PacBio (NCBI SRA accession: SRR35543630) from faecal DNA revealed a complete 16S rRNA gene with 100% identity to the corresponding sequence in the genome assembly. The 16S rRNA gene sequence is therefore considered complete. Genome completeness and contamination were assessed using CheckM2 v1.1.0. The NCBI 16S rRNA accession number of the genome sequence is PX000509 and the raw read FASTQ files are available under the accession numbers SRR35183424, SRR35215812, SRR35215811 and SRR35215810. 
Automated checks
Complete

Last modified 3 months ago

Sample Metadata

BioSample entries
Date
Not detected
Location
Not detected
Toponym
Not detected
Environment
Sample Type: metagenomic assembly
Isolation Source: gut
Env Broad Scale: Feces
Other
Host: Homo sapiens
Package
Ncbi Package: MIMAG.human-gut.6.0
All retrieved samples

Metadata retrieved 3 months ago

Sequencing Experiments

RNA baits capture of Mali 340
HiC of Mali 340
RNA baits capture of Mali 340
WGS of Mali 340: fecal DNA
amplicon PacBio 16S rRNA gene sequencing data


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