Microbiology Resource Announcements

ABSTRACT We present the complete de novo assembly of a Candidatus Walczuchella monophlebidarum genome (286,606 bp), a flavobacterial endosymbiont of the giant-scale insect Icerya purchasi . The genome was assembled from metagenomic short read Illumina sequences obtained from DNA of citrus leaves collected in Carinthia, Austria in November 2024.

ABSTRACT Leptothrix mechoopdaensis BB-3 and BB-4 are metal-oxidizing, sheathed, filamentous bacteria that were isolated from a woodland iron seep near Centerville, Chico, CA, USA. Here, we report a closed circular genome sequence for each strain, assembled from reads sequenced on an Oxford Nanopore FLO-MIN114 flow cell.

ABSTRACT Cable bacteria conduct long-distance electron transport in sediments but are not yet isolated in pure culture. We report the metagenome-assembled genome of Candidatus Electrothrix sp. NPCB-01 from Newport Bay, California. This 3.46-Mb genome encodes sulfur oxidation, nitrogen and carbon metabolism, and nickel homeostasis genes, expanding resources for these electroactive microbes.

ABSTRACT We report the draft metagenome-assembled genome (MAG) of “ Candidatus Phytoplasma australasiaticum” strain TBB-AP, obtained from a symptomatic tomato plant collected in Andhra Pradesh, India. This assembly provides a genomic resource for functional and evolutionary studies of phytoplasmas associated with tomato big bud disease.

ABSTRACT Five draft genome sequences of ‘ Candidatus Liberibacter solanacearum’ haplotype E from five different Bactericera trigonica vector insects sampled in France are reported. Each genome has a size of about 1.20 Mbp, a G + C content of 35% and approximately 1,000 predicted coding DNA sequences.

ABSTRACT Candidatus Loosdrechtia sp. KJ is an anaerobic ammonium-oxidizing bacterium enriched from a bioreactor operated under alkaline conditions (pH 9.2 ± 0.4). We report its 3.39 Mb draft genome, containing 3,065 predicted coding sequences, 47 tRNA genes, and a single rrn operon.

ABSTRACT The complete genome, 173,958 bp in size, of “ Candidatus Carsonella ruddii” DC-OKEB1 , an obligate bacterial endosymbiont of the Asian citrus psyllid Diaphorina citri , was determined. The genome sequence provides valuable information for comparative and evolutionary aspects of the intimate insect–microbe mutualism.

ABSTRACT We report the draft genomes of five Clostridium isolates from soil and agricultural by-products, four of which are proposed as Candidatus species. Members of the genus Clostridium are of significant industrial interest, and the availability of their genome sequences facilitates the understanding and exploration of their functional potential.

ABSTRACT The complete genome, 226,287 bps in size, of “ Candidatus Nardonella sp.” Pin-AIST, an obligatory bacterial endosymbiont of the black hard weevil Pachyrhynchus infernalis , was sequenced. The extremely reduced endosymbiont genome is specialized for tyrosine synthesis, which contributes to the hardness of the beetle’s exoskeleton.

ABSTRACT “Candidatus Dehalogenimonas loeffleri” strain W, isolated from estuarine sediments, can dechlorinate 1,2-dichloroethane under high salinity. Its genome consists of a circular 1,772,240-bp chromosome with a G + C content of 52.5% and encompasses 1,763 protein-coding sequences, including 28 genes encoding reductive dehalogenases.