Methylobacter titanis


Formal styling
Methylobacter titanis Roldán, Menes, 2023
Effective publication
Roldán, Menes, 2023
SeqCode status
Valid (SeqCode)
Register List (validated)
Cannonical URL


L. gen. masc. n. titanis, in reference to Titan, Saturn's moon with rivers and lakes of methane at low temperatures
Nomenclatural type
NCBI Assembly: GCA_029946125.1
Nomenclatural status
Validly published under the SeqCode


K-2018 and D1-2020 were enriched from lake sediment samples collected at Kitezh (62°11’36’’S, 58°57’58’’W) and Drake 1 (62°10’37’’S, 58°55’16’’W) lakes, respectively, in King George Island, maritime Antarctica. Cells of K-2018 MAG008 and D1-2020 MAG004(Ts) are Gram negative cocci, single or in pairs. Growth occurs at 5 to 30 ºC. The MAGs encoded the genes for particulate membrane-bound methane monooxygenase (pMMO) but not for soluble methane monooxygenase (sMMO). The genes necessary for the synthesis of tetrahydrofolate (THF) were present, but the genes for the cofactors methanefuran (MFR) and tetrahydromethanepterin (THPMT) were absent. The genes that encode the ribulose-monophosphate (RuMP) pathway for carbon fixation and the almost complete serine pathway for carbon fixation were present, but not the genes for malate-CoA ligase. The genes encoding the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (Rubisco) were not detected but the genes for the Entner-Doudoroff pathway were found, except the gene encoding a hexokinase that catalyses the phosphorylation of glucose to glucose 6-phosphate. The genes for the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway were also found. The genes for the nitrate reductase NarGHJI and the nitrite reductase NirBD were present suggesting the organism could use nitrate and nitrite as nitrogen sources. However, no genes were found that encode for urease (ureBA) or nitrogenase (nifHDK). The DNA G+C content is 47.9-49.3%. The MAG D1-2020 MAG004(Ts) is the designated nomenclatural type for the species. The GenBank/EMBL/DDBJ accession number for MAGs K-2018 MAG008 and D1-2020 MAG004(Ts) are JAQSDF000000000 and JAQSDX000000000, respectively.
Proposed as a candidate species of methanotroph of the genus Methylobacter in Antonie van Leeuwenhoek journal
Bacteria » Pseudomonadota » Gammaproteobacteria » Methylococcales » Methylococcaceae » Methylobacter » Methylobacter titanis


NCBI Assembly:GCA_029946125.1
Metagenome-Assembled Genome (MAG)
Estimated Quality Metrics
  • Completeness: 99.47%
  • Contamination: 2.04%
  • Quality: 89.27
Ribosomal and transfer RNA genes
  • 1 16S rRNA (up to 100.0%)
  • 1 23S rRNA (up to 100.0%)
  • tRNAs for 20 amino acids
Sequencing depth
521.0 ×
Other features
  • G+C Content: 47.71%
  • Coding Density: 87.63%
  • Codon Table: 11
  • N50: 51,691 bp
  • Contigs: 146
  • Largest Contig: 155,944 bp
  • Assembly Length: 3,510,200 bp
  • Ambiguous Assembly Fraction: 0.0054%
Automated checks
See additional details


Outside links and data sources
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Local history
Registered by
Roldán, Diego M. about 1 year ago
Submitted by
Roldán, Diego M. about 1 year ago
Endorsed by
Chuvochina, Maria about 1 year ago
Validated by
Chuvochina, Maria about 1 year ago
Date of priority
2023-05-26 07:23 PM (UTC)


Citation Title
Roldán, Menes, 2023, Antonie van Leeuwenhoek Characterisation of ‘Candidatus Methylobacter titanis’ sp. nov., a putative novel species of Methylobacter clade 2 and their distribution in sediments of freshwater lakes in maritime Antarctica
Effective publication

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