Methanocrinis alkalitolerans


Formal styling
Methanocrinis alkalitolerans Khomyakova et al., 2023 (valid 2024)
Effective publication
Khomyakova et al., 2023
SeqCode status
Valid (SeqCode)
Register List (validated)
Cannonical URL


N.L. neut. n. alkali, alkali; L. pres. part. tolerans, tolerating; N.L. part. adj. alkalitolerans, tolerating high alkalinity
Nomenclatural type
NCBI Assembly: GCA_029167205.1
Reference strain
Nomenclatural status
Validly published under the SeqCode


Cells are non-motile, rod-shaped, 1.7–6.5 μm in length and 0.9–1.5 μm in diameter. Can form polar pili/fimbriae-like structures of unknown nature on the surface of the cell. Filaments are formed after long incubation times. Growth occurs at 20–45°C (optimum, 37 °C) and at pH 7.5–10.0 (optimum 9.0); the presence of NaCl is not required. Yeast extract is not essential for growth, but highly stimulatory. Utilizes acetate for methane production. No growth or CH4 formation is observed on H2/CO2, formate, carbon monoxide and methanol. The complete genome of strain M04AcTs, available under the GenBank assembly accession number (GCA_029167205) is the designated nomenclatural type for the species and was recovered from an enrichment culture, cultivated on acetate and established from a terrestrial mud volcano at the Taman Peninsula, Russian Federation. The genome is characterized by a size of 2.44 Mb and a G+C content of 58.31 mol%. Completeness is estimated by CheckM at 99.84% with 0.00% contamination. The GenBank accession number for the 16S rRNA gene sequence of M04AcTs is OQ918309.
Archaea » Halobacteriota » Methanosarcinia » Methanotrichales » Methanotrichaceae » Methanocrinis » Methanocrinis alkalitolerans
Methanocrinis gtdb


NCBI Assembly:GCA_029167205.1
Enrichment Genome
Estimated Quality Metrics
  • Completeness: 99.84%
  • Contamination: 0.0%
  • Quality: 99.84
Ribosomal and transfer RNA genes
  • 1 16S rRNA (up to 100.0%)
  • 1 23S rRNA (up to 100.0%)
  • tRNAs for 18 amino acids
Sequencing depth
240.0 ×
Other features
  • G+C Content: 58.0%
  • Coding Density: 86.11%
  • Codon Table: 11
  • N50: 58,935 bp
  • Contigs: 96
  • Largest Contig: 128,298 bp
  • Assembly Length: 2,444,195 bp
  • Ambiguous Assembly Fraction: 0.0%
Submitter comments
Completeness and contamination were evaluated using CheckM (Parks et al., 2015).
Annotation was made using PGAP (Tatusova et al., 2016).
tRNA elements were detected by tRNAscan-SE (Lowe and Eddy, 1997).
Automated checks
See additional details


Outside links and data sources
Search sequences
Local history
Registered by
Merkel, Alexander 10 months ago
Submitted by
Merkel, Alexander 6 months ago
Validated by
Rodriguez-R, Luis M 5 months ago
Date of priority
2024-01-07 10:19 AM (UTC)


Citation Title
Khomyakova et al., 2023, Frontiers in Microbiology Phenotypic and genomic characterization of the first alkaliphilic aceticlastic methanogens and proposal of a novel genus Methanocrinis gen.nov. within the family Methanotrichaceae
Khomyakova et al., 2023, Frontiers in Microbiology Phenotypic and genomic characterization of Bathyarchaeum tardum gen. nov., sp. nov., a cultivated representative of the archaeal class Bathyarchaeia
Effective publication

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