Type strain is Allorhodoferax aquaticus MsE-M22 (GCA_965194715.1), isolated from 5 m depth from Lake Most, Czechia (date: 2019-07-30), via high-throughput dilution to extinction cultivation. MsE-M22 has a genome size of 3.7 Mbp with a genomic GC content of 59.3%, contains 6 rRNA genes and 47 tRNAs. The genome is complete, consisting of a circular chromosome. Genes for flagellar and pilus assembly were annotated. Pathways for nitrate reduction, cyanate and urea degradation, glycolate oxidation and the biosynthesis of all amino acids except for aspartate were predicted. Further, pathways for thiamine, riboflavin, NAD, coenzyme A, THF, cobalamin, and heme biosynthesis were identified. The closest cultivated relative is Curvibacter sp. AEP1-3 (GCF_002163715.1), with an average amino acid identity of 90.7% and average nucleotide identity of 86.6%. Current GTDB classification (R220): d__Bacteria; p__Pseudomonadota; c__Gammaproteobacteria; o__Burkholderiales; f__Burkholderiaceae; g__Rhodoferax_C; s__Rhodoferax_C sp027488985.