Pseudomonas acericola sp. nov. is a member of the Pseudomonas fluorescens subgroup, isolated from maple sap (Acer saccharum) in Quebec, Canada. The genome size is approximately 6.18 Mbp with a GC content of 61.37%. Phylogenomic analysis places this species in a distinct clade, with ANI values below 95% and dDDH values below 70% compared to all known type strains. Diagnostic genomic traits include: Presence of multiple nonribosomal peptide synthetase (NRPS) clusters for cyclic lipopeptides, Presence of quorum-sensing related homoserine lactone (HSL) clusters, with partial similarity to known polyketide and cepacin A clusters; Presence of a terpene and a microcin biosynthetic gene cluster; Absence of phenazine biosynthetic operons, distinguishing it from P. edsoni.