Publications

4868

ABSTRACT “ Candidatus Neoehrlichia mikurensis” is the tick-borne agent of neoehrlichiosis, an infectious disease that primarily affects immunocompromised patients. So far, the genetic variability of “ Ca . Neoehrlichia” has been studied only by comparing 16S rRNA genes and groEL operon sequences. We describe the development and use of a multilocus sequence analysis (MLSA) protocol to characterize the genetic diversity of clinical “ Ca . Neoehrlichia” strains in Europe and their relatedness to other species within the Anaplasmataceae family. Six genes were selected: ftsZ , clpB , gatB , lipA , groEL , and 16S rRNA. Each MLSA locus was amplified by real-time PCR, and the PCR products were sequenced. Phylogenetic trees of MLSA locus relatedness were constructed from aligned sequences. Blood samples from 12 patients with confirmed “ Ca . Neoehrlichia” infection from Sweden ( n = 9), the Czech Republic ( n = 2), and Germany ( n = 1) were analyzed with the MLSA protocol. Three of the Swedish strains exhibited identical lipA sequences, while the lipA sequences of the strains from the other nine patients were identical to each other. One of the Czech strains had one differing nucleotide in the clpB sequence from the sequences of the other 11 strains. All 12 strains had identical sequences for the genes 16S rRNA, ftsZ , gatB , and groEL . According to the MLSA, among the Anaplasmataceae , “ Ca . Neoehrlichia” is most closely related to Ehrlichia ruminantium , less so to Anaplasma phagocytophilum , and least to Wolbachia endosymbionts. To conclude, three sequence types of infectious “ Ca . Neoehrlichia” were identified: one in the west of Sweden, one in the Czech Republic, and one spread throughout Europe.

rinjal is an important vegetable crop grown worldwide. A field survey conducted during 2012 in Kerala state of South India, the little leaf disease affected brinjal plant was observed in one of the home gardens located at Nemmara (Palakkad district). The diseased and healthy samples were collected; genomic DNA was isolated and 16SrRNA gene amplified by performing nested PCR using universal primers pairs P1/P7 and R16F2n/R2 respectively. The association of Phytoplasma was detected in all the three symptomatic brinjal samples and in Phytoplasma affected Periwinkle used as positive control. An expected amplicon size of 1250bp from nested PCR was purified and sequenced. The nucleotide sequence analysis (nBLAST) of 1250bp fragment of sequence KL-50(KP027530) revealed 97% similarity with Candidatus phytoplasma trifolii (JX104336). Phylogenetic analysis and study of virtual RFLP gel pattern of the sequence also revealed the association of Candidatus phytoplasma trifolii.

Abstract Me.tha.no.bre.vi.bac'ter. M.L. neut. n. methanum methane; L. masc. adj. brevis short; M.L. masc. n. bacter equivalent of Gr. neut. n. bakterion rod, staff; M.L. masc. n. Methanobrevibacter short methane (‐producing) rod. Oval rods or cocci to short rods , usually occurring in pairs or chains; about 0.5–0.7 µm in width and 0.8–1.4 µm in length. Rarely, filaments are formed. Nonsporing, Gram‐positive. Cell walls are composed of pseudomurein . Nonmotile. Strict anaerobes. Optimum temperature, 37–40°C; maximum, ~45°C; minimum, ~30°C. The mol % G + C of the DNA is : 27.5–31.6. Type species : Methanobrevibacter ruminantium (Smith and Hungate 1958) Balch and Wolfe 1981, 216 (Effective publication: Balch and Wolfe in Balch, Fox, Magrum, Woese and Wolfe, 1979, 284) ( Methanobacterium ruminantium Smith and Hungate 1958, 717). Taxonomic and Nomenclature Notes According to the List of Prokaryotic names with Standing in Nomenclature (LPSN), the taxonomic status of the genus Methanobrevibacter is: correct name (last update, February 2025) * . LPSN classification: Archaea / Methanobacteriati / Methanobacteriota / Methanobacteria / Methanobacteriales / Methanobacteriaceae / Methanobrevibacter The genus Methanobrevibacter can also be recovered in the Genome Taxonomy Database (GTDB) as g__Methanobrevibacter (version v220) ** . GTDB classification: d__Archaea / p__Methanobacteriota / c__Methanobacteria / o__Methanobacteriales / f__Methanobacteriaceae / g__Methanobrevibacter * Meier‐Kolthoff et al. ( 2022 ). Nucleic Acids Res , 50 , D801 – D807 ; DOI: 10.1093/nar/gkab902 ** Parks et al. ( 2022 ). Nucleic Acids Res , 50 , D785 – D794 ; DOI: 10.1093/nar/gkab776

Abstract Fri'tsche.a. N.L. fem. n. Fritschea named after Thomas R. Fritsche, an American physician and parasitologist, in honor of his contributions to our current knowledge on chlamydial diversity. Coccoid to rod‐shaped, nonmotile, obligately intracellular bacteria, up to 2.5 μm in length . Cells show a developmental cycle with morphologically distinct stages similar to those of the Chlamydiaceae . Organisms were found in whitefly and scale insects, but have not yet been obtained in cell culture. The genus is based on the description of “ Candidatus Fritschea bemisiae” and “ Candidatus Fritschea eriococci” (Everett et al., 2005; Thao et al., 2003) (Figure 1). Type species : “ Candidatus Fritschea bemisiae ” Everett, Thao, Horn, Dyszynski and Baumann 2005, 1585. Taxonomic and Nomenclature Notes According to the List of Prokaryotic names with Standing in Nomenclature (LPSN), the taxonomic status of the genus Candidatus Fritschea is: preferred name (not correct name) (last update, February 2025) * . LPSN classification: Bacteria / Pseudomonadati / Chlamydiota / Chlamydiia / Chlamydiales / Simkaniaceae / Candidatus Fritschea Candidatus Fritschea could not be recovered in GTDB ** . * Meier‐Kolthoff et al. ( 2022 ). Nucleic Acids Res , 50 , D801 – D807 ; DOI: 10.1093/nar/gkab902 ** Parks et al. ( 2022 ). Nucleic Acids Res , 50 , D785 – D794 ; DOI: 10.1093/nar/gkab776

Abstract Bro.ca.di.a'les. N.L. fem. n. “ Candidatus Brocadia” type genus of the order; ‐ ales ending to denote an order; N.L. fem. pl. n. Brocadiales the order of “ Candidatus Brocadia”. Taxonomic and Nomenclature Notes According to the List of Prokaryotic names with Standing in Nomenclature (LPSN), the taxonomic status of the order Candidatus Brocadiales is: preferred name (not correct name) (last update, February 2025) * . LPSN classification: Bacteria / Pseudomonadati / Planctomycetota / Candidatus Brocadiia / Candidatus Brocadiales The order Candidatus Brocadiales can also be recovered in the Genome Taxonomy Database (GTDB) as o__Brocadiales (version v220) ** . GTDB classification: d__Bacteria / p__Planctomycetota / c__Brocadiia / o__Brocadiales * Meier‐Kolthoff et al. ( 2022 ). Nucleic Acids Res , 50 , D801 – D807 ; DOI: 10.1093/nar/gkab902 ** Parks et al. ( 2022 ). Nucleic Acids Res , 50 , D785 – D794 ; DOI: 10.1093/nar/gkab776