Publications

4481

DNA from low-biodiversity fracture water collected at 2.8-kilometer depth in a South African gold mine was sequenced and assembled into a single, complete genome. This bacterium, Candidatus Desulforudis audaxviator , composes >99.9% of the microorganisms inhabiting the fluid phase of this particular fracture. Its genome indicates a motile, sporulating, sulfate-reducing, chemoautotrophic thermophile that can fix its own nitrogen and carbon by using machinery shared with archaea. Candidatus Desulforudis audaxviator is capable of an independent life-style well suited to long-term isolation from the photosphere deep within Earth's crust and offers an example of a natural ecosystem that appears to have its biological component entirely encoded within a single genome.

Symptoms resembling “zebra chip” disease (3) were observed in potato (Solanum tuberosum) tubers harvested from a breeding trial in South Auckland, New Zealand in May 2008. The tubers had necrotic flecking and streaking that became marked when the potatoes were fried. Affected plants generally senesced early, at the beginning of April. The mean yield was approximately 60% less than expected and harvested tubers had less dry matter (13%) than normal (19%). Large numbers of the psyllid Bactericera cockerelli were observed on the crop during the summer. Total DNA was extracted from the vascular tissue of five symptomatic tubers and seven volunteers collected from the affected field with a DNeasy Plant Mini Kit (Qiagen, Valencia, CA). Samples were tested by PCR using primers OA2 (GenBank Accession No. EU834130) and OI2c (2). These primers amplify a 1,160-bp fragment of the 16S rRNA sequence of a ‘Candidatus Liberibacter’ species identified in tomato and capsicum in New Zealand. No fragment was amplified from healthy plants, but amplicons of the expected size were obtained from all symptomatic tubers and one plant. A 650-bp fragment of the β operon was also amplified from symptomatic tubers. The amplicons were directly sequenced (GenBank Accession Nos. EU849020 and EU919514). BLAST analysis showed 100% identity to the tomato/capsicum liberibacter (GenBank Accession Nos. EU834130 and EU834131). From a commercial potato field adjoining the breeding trial, groundkeeper tubers were collected and separated into those that were asymptomatic and those that exhibited a range of symptoms. Total DNA was extracted and tested by PCR using the OA2/OI2c primers. In the first category, 6 of 10 tubers tested positive, whereas the 10 tubers in the second category tested negative. Two phytoplasmas seem to be involved in the “zebra chip” disease complex (4) but were not detected in the samples in this study. To our knowledge, this is the first report of a liberibacter associated with disease in potato. From transmission electron microscope observations, previous researchers have hypothesized that a bacterium-like organism may cause “zebra chip” (1) and B. cockerelli is associated with the disease (3). “Zebra chip” was first reported in Mexico in 1994, since then it has caused significant economic damage in Guatemala, Mexico, and the southwestern United States. The economic impact of the disease in New Zealand is yet to be determined. References: (1) S. H. De Boer et al. Page 30 in: New and Old Pathogens of Potato in Changing Climate. A. Hannukkala and M. Segerstedt, eds. Online publication. Agrifood Research Working Paper 142, 2007. (2) S. Jagoueix et al. Mol. Cell. Probes 10:43, 1996. (3) J. E. Munyaneza et al. J. Econ. Entomol. 100:656, 2007. (4) G. A. Secor et al. Plant Dis. 90:377, 2006.

ABSTRACT “ Candidatus Midichloria mitochondrii,” an intracellular symbiont of the tick Ixodes ricinus , is the only described organism able to invade the mitochondria of any multicellular organism. We used quantitative PCR to examine cycles of bacterial growth and death throughout the host's development and found that they correspond with the phases of engorgement and molt, respectively.

Summary Members of the uncultured bacterial genus Candidatus Accumulibacter are capable of intracellular accumulation of inorganic phosphate in activated sludge wastewater treatment plants (WWTPs) performing enhanced biological phosphorus removal, but were also recently shown to inhabit freshwater and estuarine sediments. Additionally, metagenomic sequencing of two bioreactor cultures enriched in Candidatus Accumulibacter, but housed on separate continents, revealed the potential for global dispersal of particular Candidatus Accumulibacter strains, which we hypothesize is facilitated by the ability of Candidatus Accumulibacter to persist in environmental habitats. In the current study, we used sequencing of a phylogenetic marker, the ppk 1 gene, to characterize Candidatus Accumulibacter populations in diverse environments, at varying distances from WWTPs. We discovered several new lineages of Candidatus Accumulibacter which had not previously been detected in WWTPs, and also uncovered new diversity and structure within previously detected lineages. Habitat characteristics were found to be a key determinant of Candidatus Accumulibacter lineage distribution while, as predicted, geographic distance played little role in limiting dispersal on a regional scale. However, on a local scale, enrichment of particular Candidatus Accumulibacter lineages in WWTP appeared to impact local environmental populations. These results provide evidence of ecological differences among Candidatus Accumulibacter lineages.

ABSTRACT A new huanglongbing (HLB) “ Candidatus Liberibacter” species is genetically characterized, and the bacterium is designated “ Candidatus Liberibacter psyllaurous.” This bacterium infects the psyllid Bactericera cockerelli and its solanaceous host plants potato and tomato, potentially resulting in “psyllid yellowing.” Host plant-dependent HLB transmission and variation in psyllid infection frequencies are found.

Feline haemoplasma infection can cause haemolytic anaemia. The natural method of transmission of haemoplasmas between cats is currently unknown but the nature of some of the risk factors for infection suggests that saliva may act as a mode of transmission. The aim of this study was to determine if Mycoplasma haemofelis (Mhf) and ‘ Candidatus Mycoplasma haemominutum’ (CMhm) DNAs could be amplified from saliva and salivary gland samples collected from haemoplasma-infected cats.