Plant Science


Publications
825

Strain Tracking of ‘Candidatus Liberibacter asiaticus’, the Citrus Greening Pathogen, by High-Resolution Microbiome Analysis of Asian Citrus Psyllids

Citation
Higgins et al. (2022). Phytopathology® 112 (11)
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid, Diaphorina citri, is an invasive insect and a vector of ‘ Candidatus Liberibacter asiaticus’ ( CLas), a bacterium whose growth in Citrus species results in huanglongbing (HLB), also known as citrus greening disease. Methods to enrich and sequence CLas from D. citri often rely on biased genome amplification and nevertheless contain significant quantities of host DNA. To overcome these hurdles, we developed a simple pretreatment DNase and filtration (PDF) protocol to rem

Genome-Informed Design of a LAMP Assay for the Specific Detection of the Strain of ‘Candidatus Phytoplasma asteris’ Phytoplasma Occurring in Grapevines in South Africa

Citation
Alič et al. (2022). Plant Disease 106 (11)
Names
Ca. Phytoplasma Ca. Phytoplasma asteri
Abstract
Grapevine yellows is one of the most damaging phytoplasma-associated diseases worldwide. It is linked to several phytoplasma species, which can vary regionally due to phytoplasma and insect-vector diversity. Specific, rapid, and reliable detection of the grapevine yellows pathogen has an important role in phytoplasma control. The purpose of this study was to develop and validate a specific loop-mediated isothermal amplification (LAMP) assay for detection of a distinct strain of grapevine ‘Candi

A “Candidatus Liberibacter asiaticus”-secreted polypeptide suppresses plant immune responses in Nicotiana benthamiana and Citrus sinensis

Citation
Shen et al. (2022). Frontiers in Plant Science 13
Names
Ca. Liberibacter asiaticus
Abstract
Citrus Huanglongbing (HLB), known as the most economically devastating disease in citrus industry, is mainly caused by phloem-restricted Gram-negative bacterium “Candidatus Liberibacter asiaticus” (CLas). To date, CLas is still unculturable in vitro, which has been dramatically delaying the research on its pathogenesis, and only few Sec-dependent effectors (SDEs) have been identified to elucidate the pathogenesis of CLas. Here, we confirmed that a CLas-secreted Sec-dependent polypeptide, namely

Candidatus Liberibacter asiaticus accumulation in the phloem inhibits callose and reactive oxygen species

Citation
Bernardini et al. (2022). Plant Physiology 190 (2)
Names
Ca. Liberibacter asiaticus
Abstract
CLas inhibits callose deposition in the sieve pores and the accumulation of reactive oxygen species to favor its cell-to-cell movement.

Candidatus Phytoplasma Australasia Associated with Alfalfa Witches’ Broom: Symptomatology, Quantitative Loss, Qualitative Loss and Molecular Characterization

Citation
Mahesha et al. (2022). LEGUME RESEARCH - AN INTERNATIONAL JOURNAL (Of)
Names
Ca. Phytoplasma Ca. Phytoplasma australasia
Abstract
Background: Alfalfa, Medicago sativa L. is the most important and widely grown leguminous fodder crop in temperate and tropical regions of the world. The production of alfalfa crop is limited by several biotic stresses, among which witches’ broom disease (AWB) was reported to cause significant economic losses. Methods: The phytoplasma infected alfalfa plants were collected from a central research farm, ICAR-IGFRI, Jhansi, U.P. Qualitative parameters such as crude protein content, acid detergent