Ecology, Evolution, Behavior and Systematics

AbstractPathogens which need a vector for their transmission can alter the vectors’ behaviour to favour their spread. We used the electrical penetration graph technique to investigate this hypothesis by using the tomato potato psyllidBactericera cockerelliinfected or not with the plant pathogenCandidatusLiberibacter solanacearum (CLso) on African boxthorn and tomato. Probing was not affected by the host type but there was a significant effect on probing due to the infection status of the psyllid. More psyllids carried out probing activities in the sieve elements when infected with CLso, and more probing activities were observed from CLso-infected psyllids by comparison to the non-infected groups. Specifically, significant increases in salivation, phloem ingestion and number of probes, before and after reaching the sieve elements, were noticed in the infected groups. Furthermore, time elapsed to reach the sieve elements was significantly shortened by 2 h in the infected group. Remaining probing activities in xylem tissues were not different between all psyllid groups. The observed changes in feeding behaviour by pathogen-infected psyllids may well ensure further spread of the pathogen as greater salivation has the potential to increase transmission, highlighting at the same time the important role that crop and non-crop hosts play in disease epidemiology.

The members of the genusFrankiaare, with a few exceptions, a group of nitrogen-fixing symbiotic actinobacteria that nodulate mostly woody dicotyledonous plants belonging to three orders, eight families and 23 genera of pioneer dicots. These bacteria have been characterized phylogenetically and grouped into four molecular clusters. One of the clusters, cluster 1 contains strains that induce nodules onAlnusspp. (Betulaceae),Myricaspp.,Morellaspp. andComptoniaspp. (Myricaceae) that have global distributions. Some of these strains produce not only hyphae and vesicles, as other cluster 1 strains do, but also numerous sporangia in their host symbiotic tissues, hence their phenotype being described as spore-positive (Sp+). While Sp+ strains have resisted repeated attempts at cultivation, their genomes have recently been characterized and found to be different from those of all described species, being markedly smaller than their phylogenetic neighbours. We thus hereby propose to create a 'CandidatusFrankia alpina' species for some strains present in nodules ofAlnus alnobetulaandA. incanathat grow in alpine environments at high altitudes or in subarctic environments at high latitudes.

The psyllids Cacopsylla melanoneura and Cacopsylla picta reproduce on apple (Malus × domestica) and transmit the bacterium ‘Candidatus Phytoplasma mali’, the causative agent of apple proliferation. Adult psyllids were collected by the beating-tray method from lower and upper parts of the apple tree canopy in the morning and in the afternoon. There was a trend of catching more emigrant adults of C.melanoneura in the morning and in the lower part of the canopy. For C.melanoneura remigrants, no differences were observed. The findings regarding the distribution of adults were reflected by the number of nymphs collected by wash-down sampling. The density of C.picta was too low for a statistical analysis. The vector monitoring and how it is commonly performed, is suitable for estimating densities of C.melanoneura. Nevertheless, above a certain temperature threshold, prediction of C.melanoneura density might be skewed. No evidence was found that other relatively abundant psyllid species in the orchard, viz. Baeopelma colorata, Cacopsylla breviantennata, Cacopsylla brunneipennis, Cacopsylla pruni and Trioza urticae, were involved in ‘Candidatus Phytoplasma mali’ transmission. The results of our study contribute to an advanced understanding of insect vector behavior and thus have a practical impact for an improved field monitoring.

The bacterial pathogen ‘Candidatus Liberibacter solanacearum’ (Lso) is transmitted by the tomato potato psyllid (TPP), Bactericera cockerelli, to solanaceous crops. In the present study, the changes in metabolic profiles of insect-susceptible (cv CastleMart) and resistant (RIL LA3952) tomato plants in response to TPP vectoring Lso or not, were examined after 48 h post infestation. Non-volatile and volatile metabolites were identified and quantified using headspace solid-phase microextraction equipped with a gas chromatograph-mass spectrometry (HS-SPME/GC-MS) and ultra-high pressure liquid chromatography coupled to electrospray quadrupole time-of-flight mass spectrometry (UPLC/ESI-HR-QTOFMS), respectively. Partial least squares-discriminant analysis (PLS-DA) was used to define the major uncorrelated metabolite components assuming the treatments as the correlated predictors. Metabolic changes in various classes of metabolites, including volatiles, hormones, and phenolics, were observed in resistant and susceptible plants in response to the insects carrying the pathogen or not. The results suggest the involvement of differentially regulated and, in some cases, implicates antagonistic metabolites in plant defensive signaling. Upon validation, the identified metabolites could be used as markers to screen and select breeding lines with enhanced resistance to reduce economic losses due to the TPP-Lso vector-pathogen complex in Solanaceous crops.

Abstract An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Summary Recent advances in sequencing technology and bioinformatic pipelines have allowed unprecedented access to the genomes of yet‐uncultivated microorganisms from diverse environments. However, the catalogue of freshwater genomes remains limited, and most genome recovery attempts in freshwater ecosystems have only targeted specific taxa. Here, we present a genome recovery pipeline incorporating iterative subtractive binning, and apply it to a time series of 100 metagenomic datasets from seven connected lakes and estuaries along the Chattahoochee River (Southeastern USA). Our set of metagenome‐assembled genomes (MAGs) represents >400 yet‐unnamed genomospecies, substantially increasing the number of high‐quality MAGs from freshwater lakes. We propose names for two novel species: ‘ Candidatus Elulimicrobium humile’ (‘ Ca . Elulimicrobiota’, ‘Patescibacteria’) and ‘ Candidatus Aquidulcis frankliniae’ (‘Chloroflexi’). Collectively, our MAGs represented about half of the total microbial community at any sampling point. To evaluate the prevalence of these genomospecies in the chronoseries, we introduce methodologies to estimate relative abundance and habitat preference that control for uneven genome quality and sample representation. We demonstrate high degrees of habitat‐specialization and endemicity for most genomospecies in the Chattahoochee lakes. Wider ecological ranges characterized smaller genomes with higher coding densities, indicating an overall advantage of smaller, more compact genomes for cosmopolitan distributions.

AbstractPhytoplasmas are specialized small bacteria restricted to the phloem tissue and spread by hemipterans feeding on plant sieve tube elements. As for many other plant pathogens, it is known that phytoplasmas alter the chemistry of their hosts. Most research on phytoplasma-plant interactions focused on the induction of plant volatiles and phytohormones. Little is known about the influence of phytoplasma infections on the nutritional composition of phloem and consequences on vector behavior and development. The plum psyllidCacopsylla prunitransmits ‘CandidatusPhytoplasma prunorum’, the causing agent of European Stone Fruit Yellows (ESFY). While severalPrunusspecies are susceptible for psyllid feeding, they show different responses to the pathogen. We studied the possible modulation of plant-insect interactions by bacteria-induced changes in phloem sap chemistry. Therefore, we sampled phloem sap from phytoplasma-infected and non-infectedPrunus persicaandPrunus insititiaplants, which differ in their susceptibility to ESFY and psyllid feeding. Furthermore, the feeding behavior and development ofC. pruninymphs was compared on infected and non-infectedP. persicaandP. insititiaplants. Phytoplasma infection did not affect phloem consumption byC. pruninymphs nor their development time. In contrast, the study revealed significant differences betweenP. insititiaandP. persicain terms of both phloem chemistry and feeding behavior ofC. pruninymphs.Phloem feeding phases were four times longer onP. insititiathan onP. persica, resulting in a decreased development time and higher mortality of vector insects onP. persicaplants. These findings explain the low infestation rates of peach cultivars with plum psyllids commonly found in field surveys.