Agronomy and Crop Science


Publications
763

The Impact of Diaphorina citri-Vectored ‘Candidatus Liberibacter asiaticus’ on Citrus Metabolism

Citation
Padhi et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’ is associated with the devastating citrus disease Huanglongbing (HLB). It is transmitted by grafting infected material to healthy plants and by the feeding of the Asian citrus psyllid (Diaphorina citri). Previously, we demonstrated that a metabolomics approach using proton-nuclear magnetic resonance spectroscopy discriminates healthy from diseased plants via grafting. This work assessed the capability of this technology in discriminating healthy and diseased

Generous Hosts: ‘Candidatus Liberibacter asiaticus’ Growth in Madagascar Periwinkle (Catharanthus roseus) Highlights Its Nutritional Needs

Citation
Killiny (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
‘Candidatus Liberibacter asiaticus’, the putative causal agent of citrus greening, is not available in pure culture yet. In addition to trees of citrus and citrus relatives, ‘Ca. L. asiaticus’ can grow in Madagascar periwinkle (Catharanthus roseus). Using gas chromatography-mass spectrometry, we compared the phloem sap composition in sweet orange ‘Valencia’ (Citrus sinensis) and periwinkle plants after the infection with ‘Ca. L. asiaticus’. Interestingly, in contrast to our previous studies of

Effect of Plant Age, Temperature, and Vector Load on ‘Candidatus Liberibacter solanacearum’ in Planta Titer and Shoot Proliferation Symptoms in Carrot

Citation
Keshet-Sitton et al. (2022). Phytopathology® 112 (1)
Names
“Liberibacter solanacearum” Ca. Phytoplasma
Abstract
A decade ago, shoot proliferation symptoms (i.e., witches’ broom) in carrots were believed to be the cause of ‘Candidatus Phytoplasma’ and Spiroplasma infection, yet in recent years this association appeared to have weakened, and a closer association was found with the yet-unculturable, psyllid-transmitted Gram-negative bacterium ‘Candidatus Liberibacter solanacearum’. In Israel, carrots are grown throughout the year, yet shoot proliferation symptoms tend to appear only in mature plants and mos

An Excised Leaf Assay to Measure Acquisition of ‘Candidatus Liberibacter asiaticus’ by Psyllids Associated with Citrus Huanglongbing Disease

Citation
Igwe et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
Huanglongbing, or citrus greening disease, is the most serious disease of citrus worldwide and is associated with plant infection by ‘Candidatus Liberibacter asiaticus’ (CLas) and other Liberibacter species. CLas is transmitted by Diaphorina citri, the Asian citrus psyllid, in a circulative propagative manner. Circulative propagative transmission is a complex process comprising at least three steps: movement of the pathogen into vector tissues, translocation and replication of the pathogen with

A Review of the ‘Candidatus Liberibacter africanus’ Citrus Pathosystem in Africa

Citation
da Graça et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter africanus
Abstract
It has been nearly 100 years since citrus growers in two distinct regions in the northern provinces of South Africa noticed unusual symptoms in their citrus trees, causing significant crop losses. They had no idea that these symptoms would later become part of an almost global pandemic of a disease called greening or huanglongbing (HLB). The rapid spread of the disease indicated that it might be caused by a transmissible pathogen, but it took >50 years to identify the causative agent as ‘Can

Candidatus Liberibacter: From Movement, Host Responses, to Symptom Development of Citrus Huanglongbing

Citation
Pandey et al. (2022). Phytopathology® 112 (1)
Names
Liberibacter
Abstract
Candidatus Liberibacter spp. are fastidious α-proteobacteria that cause multiple diseases on plant hosts of economic importance, including the most devastating citrus disease: Huanglongbing (HLB). HLB was reported in Asia a century ago but has since spread worldwide. Understanding the pathogenesis of Candidatus Liberibacter spp. remains challenging as they are yet to be cultured in artificial media and infect the phloem, a sophisticated environment that is difficult to manipulate. Despite those

Detection and Identification of a ‘Candidatus Liberibacter solanacearum’ Species from Ash Tree Infesting Psyllids

Citation
Wamonje et al. (2022). Phytopathology® 112 (1)
Names
“Liberibacter solanacearum” Liberibacter
Abstract
‘Candidatus Liberibacter’ species are associated with severe, economically important diseases. Nearly all known species are putatively insect transmitted, specifically by psyllids. Detection of ‘Ca. Liberibacter’ in plants is complicated by their uneven distribution in host plants and largely fastidius nature. The death of black (Fraxinus nigra) and mancana (Fraxinus mandshurica) ash trees in Saskatchewan, Canada has been associated with infestation by the cottony ash psyllid (Psyllopsis discre

Prevalent Transmission of ‘Candidatus Liberibacter asiaticus’ over ‘Ca. Liberibacter americanus’ in a Long-Term Controlled Environment

Citation
Gasparoto et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter americanus Ca. Liberibacter asiaticus
Abstract
In Brazil, citrus huanglongbing (HLB) is associated with ‘Candidatus Liberibacter americanus’ (CLam) and ‘Ca. Liberibacter asiaticus’ (CLas). However, there are few studies about HLB epidemiology when both Liberibacter spp. and its insect vector, the Asian citrus psyllid (ACP, Diaphorina citri), are present. The objective of this work was to compare the transmission of HLB by ACP when both CLam and CLas are present as primary inoculum. Two experiments were performed under screenhouse conditions

Host Plant Adaptation Drives Changes in Diaphorina citri Proteome Regulation, Proteoform Expression, and Transmission of ‘Candidatus Liberibacter asiaticus’, the Citrus Greening Pathogen

Citation
Ramsey et al. (2022). Phytopathology® 112 (1)
Names
Ca. Liberibacter asiaticus
Abstract
The Asian citrus psyllid (Diaphorina citri) is a pest of citrus and the primary insect vector of the bacterial pathogen, ‘Candidatus Liberibacter asiaticus’ (CLas), which is associated with citrus greening disease. The citrus relative Murraya paniculata (orange jasmine) is a host plant of D. citri but is more resistant to CLas compared with all tested Citrus genotypes. The effect of host switching of D. citri between Citrus medica (citron) and M. paniculata plants on the acquisition and transmi