Publications

4485

Summary A hallmark of the SUP05 clade of marine Gammaproteobacteria is the ability to use energy obtained from reduced inorganic sulfur to fuel autotrophic fixation of carbon using RuBisCo. However, some SUP05 also have the genetic potential for heterotrophic growth, raising questions about the roles of SUP05 in the marine carbon cycle. We used genomic reconstructions, physiological growth experiments and proteomics to characterize central carbon and energy metabolism in Candidatus Thioglobus singularis strain PS1, a representative from the SUP05 clade that has the genetic potential for autotrophy and heterotrophy. Here, we show that the addition of individual organic compounds and 0.2 μm filtered diatom lysate significantly enhanced the growth of this bacterium. This positive growth response to organic substrates, combined with expression of a complete TCA cycle, heterotrophic pathways for carbon assimilation, and methylotrophic pathways for energy conversion demonstrate strain PS1's capacity for heterotrophic growth. Further, our inability to verify the expression of RuBisCO suggests that carbon fixation was not critical for growth. These results highlight the metabolic diversity of the SUP05 clade that harbours both primary producers and consumers of organic carbon in the oceans and expand our understanding of specific pathways of organic matter oxidation by the heterotrophic SUP05.

A 4-year-old male one-humped camel (Camelus dromedarius) was referred to Veterinary Teaching Hospital of Urmia University, Iran in 2017 with anorexia, weakness, depression and pale mucosa. Decreased red blood cell count, packed cell volume and hemoglobin concentration were detected by complete blood cell count. In Giemsa-stained peripheral blood smears Trypanosoma spp. trypomastigotes scattered between erythrocytic spaces and Mycoplasma-like organisms were observed attached to the surface of erythrocytes. Species-specific PCR assay confirmed T. evansi and Candidatus Mycoplasma haemolamae (CMhl) co-infection. Administration of diminazene aceturate, oxytetracycline 20%, flunixin meglumine and phosphorus-vitamin B12 were not effective in treatment. Hemoplasmosis should be considered as an important differential diagnosis of conditions associated with hemolytic anemia in camel.

Candidatus Liberibacter solanacearum (CLso) es una bacteria fitopatógena Gram-negativa, limitada al floema en solanáceas y no cultivable in vitro. Es transmitida de manera vertical y horizontal por el psílido Bactericera cockerelli. En México se asocia como responsable de la enfermedad "permanente del tomate", "punta morada de la papa" (Zebra chip) y "variegado del chile". Los síntomas causados por la bacteria varían según el cultivar y la etapa de crecimiento del hospedante pero consisten principalmente en amarillamientos y deformación de la lámina foliar, debido a la alimentación del vector y la colonización del patógeno. Las infecciones ocasionadas por CLso reducen la calidad del producto y el valor comercial en el mercado. La presencia de esta bacteria ha sido detectada en los estados de Coahuila, Sinaloa y Guanajuato, México a través de técnicas moleculares; mientras que el control de la enfermedad se encuentra enfocado en el vector, mediante prácticas culturales y la aplicación de agentes químicos y biológicos. Por lo anterior el objetivo del trabajo es puntualizar la situación actual de la distribución de CLso en México, los métodos de diagnóstico y las estrategias para el manejo integrado de la enfermedad y el vector.

Background: Tick-borne diseases are frequent in the Southeastern section of Brazil. The most prevalent canine parasites diagnosed are Ehrlichia canis, Babesia gibsoni, Babesia canis and Anaplasma platys, although Mycoplasma haemocanis and candidatus Mycoplasma haematoparvum have been registered in the country. Methods: When two clinically healthy dogs from a suburban area of the state of Rio de Janeiro, Brazil with history of being heavily infested with ticks were examined at Idexx Reference Laboratories, California for tick panel check. Results: One dog harbored DNA of candidatus Mycoplasma haematoparvum and the other DNA of Mycoplasma haemocanis. Conclusions: These results suggest that monitoring for tick infestations and tick-borne parasites must be permanent in southeastern Brazil, especially due to global climate changes which may contribute to spread and increase the number of infections in the Country.