Publications

4364

Blood samples from 426 healthy and sick cats in the UK were tested in a PCR assay for ‘Candidatus Mycoplasma haemominutum’ and Mycoplasma haemofelis (basonym Haemobartonella felis ). Seventy‐two of the cats (16·9 per cent) were positive for ‘Candidatus M haemominutum’ alone, six (1·4 per cent) were positive for M haemofelis alone and one (0·2 per cent) was positive for both. Logistic regression analysis indicated that older male cats were significantly more likely to be infected with ‘Candidatus M haemominutum’, but there was no significant association between it and any of the haematological variables measured. M haemofelis infection was uncommon in the anaemic cats sampled, and there were too few positive cases for multivariable analysis to be performed for M haemofelis ‐positive status.

ABSTRACT We report on a new Actinobaculum species, “ Actinobaculum massiliae ,” isolated from the urine of an elderly woman with recurrent cystitis. Its phenotypic pattern was similar to those of both of the other Actinobaculum species described to date. On 16S rRNA sequencing, the Marseille isolate shared 95% homology with Actinobaculum suis , 92 to 93% homology with Actinobaculum schaalii , 91 to 92% homology with Arcanobacterium spp., and 87 to 90% homology with Actinomyces species. A bootstrap value of 99% supports the node separating the Actinobaculum sp. from its closest neighbor ( A. suis ). In conclusion, on the basis of phenotypic, genotypic, and phylogenetic assessments, we show that the Marseille isolate is a previously unrecognized organism within the Actinobaculum genus, and we propose placement of the organism in the taxon “ Actinobaculum massiliae .”