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The metagenomic landscape of a high‐altitude geothermal spring in Tajikistan reveals a novel Desulfurococcaceae member, Zestomicrobium tamdykulense gen. nov., sp. nov

Citation
Dzhuraeva et al. (2024). MicrobiologyOpen 13 (5)
Names
Abstract
AbstractMetagenomic analysis was conducted to assess the microbial community in the high‐altitude Tamdykul geothermal spring in Tajikistan. This analysis yielded six high‐quality bins from the members of Thermaceae, Aquificaceae, and Halothiobacillaceae, with a 41.2%, 19.7%, and 18.1% share in the total metagenome, respectively. Minor components included Schleiferia thermophila (1.6%) and members of the archaeal taxa Pyrobaculum (1.2%) and Desulfurococcaceae (0.7%). Further analysis of the metag

Development of a TaqMan Assay for the Detection of ‘Candidatus Phytoplasma brasiliense’ and Assessment by High-Resolution Melt Curve Analysis

Citation
Lane et al. (2024). Plant Disease 108 (10)
Names
Ca. Phytoplasma brasiliense
Abstract
‘Candidatus Phytoplasma brasiliense’ (CPB) is a phytoplasma originally discovered in South America and is known to infect a wide variety of economically important crops. It is most prevalent in Hibiscus spp., where it causes witches broom symptoms, and papaya, where it causes bunchy top. Recently, CPB was documented for the first time in North America in a new host, globe sedge. In this study, two quantitative PCR assays are developed: one using high-resolution melt curve analysis (HRMA) based

Multiplex qPCR for the detection of bacteria associated with huanglongbing 'Candidatus Liberibacter asiaticus', 'Ca. L. americanus' and 16Sr IX group phytoplasma

Citation
Martins et al. (2024). Plant Disease
Names
Ca. Phytoplasma Ca. Liberibacter asiaticus Liberibacter
Abstract
The occurrence of ‘Candidatus Liberibacter’ spp. and ‘Ca. Phytoplasma’ spp. associated with blotchy mottle symptoms poses challenges to huanglongbing (HLB) diagnosis using molecular techniques. The ability to detect multiple targets simultaneously and specifically is a key aspect met by qPCR. A set of primers and hydrolysis probes useful either in single or multiplex reactions for the detection and quantification of HLB-associated bacteria were developed. Sequences from conserved genes of the r

Unique episymbiotic relationship between Candidatus Patescibacteria and Zoogloea in activated sludge flocs at a municipal wastewater treatment plant

Citation
Fujii et al. (2024). Environmental Microbiology Reports 16 (5)
Names
Ca. Patescibacteria
Abstract
AbstractCandidatus Patescibacteria, also known as candidate phyla radiation (CPR), including the class‐level uncultured clade JAEDAM01 (formerly a subclass of Gracilibacteria/GN02/BD1‐5), are ubiquitous in activated sludge. However, their characteristics and relationships with other organisms are largely unknown. They are believed to be episymbiotic, endosymbiotic or predatory. Despite our understanding of their limited metabolic capacity, their precise roles remain elusive due to the difficulty

Whole cell affinity for 4‐amino‐5‐hydroxymethyl‐2‐methylpyrimidine (<scp>HMP</scp>) in the marine bacterium Candidatus<scp>Pelagibacter</scp> st. <scp>HTCC7211</scp> explains marine dissolved <scp>HMP</scp> concentrations

Citation
Brennan et al. (2024). Environmental Microbiology Reports 16 (5)
Names
Ca. Pelagibacter
Abstract
AbstractVitamin B1 is a universally required coenzyme in carbon metabolism. However, most marine microorganisms lack the complete biosynthetic pathway for this compound and must acquire thiamin, or precursor molecules, from the dissolved pool. The most common version of Vitamin B1 auxotrophy is for thiamin's pyrimidine precursor moiety, 4‐amino‐5‐hydroxymethyl‐2‐methylpyrimidine (HMP). Frequent HMP auxotrophy in plankton and vanishingly low dissolved concentrations (approximately 0.1–50 pM) sugg