Biotechnology


Publications
188

Involvement of Rhodocyclus -Related Organisms in Phosphorus Removal in Full-Scale Wastewater Treatment Plants

Citation
Zilles et al. (2002). Applied and Environmental Microbiology 68 (6)
Names
“Accumulibacter”
Abstract
ABSTRACT The participation of organisms related to Rhodocyclus in full-scale enhanced biological phosphorus removal (EBPR) was investigated. By using fluorescent in situ hybridization techniques, the communities of Rhodocyclus -related organisms in two full-scale wastewater treatment plants were estimated to represent between 13 and 18% of the total bacterial population. However, the fractions of these communities that partic

Evidence for the Biosynthesis of Bryostatins by the Bacterial Symbiont “ Candidatus Endobugula sertula” of the Bryozoan Bugula neritina

Citation
Davidson et al. (2001). Applied and Environmental Microbiology 67 (10)
Names
“Endobugula sertula”
Abstract
ABSTRACT The marine bryozoan, Bugula neritina , is the source of the bryostatins, a family of macrocyclic lactones with anticancer activity. Bryostatins have long been suspected to be bacterial products. B. neritina harbors the uncultivated gamma proteobacterial symbiont “ Candidatus Endobugula sertula.” In this work several lines of evidence are presented that show that the symbiont is the most likely

Cloning of the spoT Gene of “ Candidatus Phlomobacter fragariae” and Development of a PCR-Restriction Fragment Length Polymorphism Assay for Detection of the Bacterium in Insects

Citation
Foissac et al. (2000). Applied and Environmental Microbiology 66 (8)
Names
Ca. Phlomobacter fragariae
Abstract
ABSTRACT Marginal chlorosis is a new disease of strawberry in which the uncultured phloem-restricted proteobacterium “ Candidatus Phlomobacter fragariae” is involved. In order to identify the insect(s) vector(s) of this bacterium, homopteran insects have been captured. Because a PCR test based on the 16S rRNA gene (rDNA) applied to these insects was unable to discriminate between “P. fragariae” and other insect-associated proteobacteria, isolation o

Cospeciation of Psyllids and Their Primary Prokaryotic Endosymbionts

Citation
Thao et al. (2000). Applied and Environmental Microbiology 66 (7)
Names
Ca. Carsonella Ca. Carsonella ruddii
Abstract
ABSTRACT Psyllids are plant sap-feeding insects that harbor prokaryotic endosymbionts in specialized cells within the body cavity. Four-kilobase DNA fragments containing 16S and 23S ribosomal DNA (rDNA) were amplified from the primary (P) endosymbiont of 32 species of psyllids representing three psyllid families and eight subfamilies. In addition, 0.54-kb fragments of the psyllid nuclear gene wingless were also amplified from 26 species. Phylogeneti

Small-subunit rRNA genes and in situ hybridization with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of "Candidatus endobugula sertula"

Citation
Haygood, Davidson (1997). Applied and Environmental Microbiology 63 (11)
Names
“Endobugula glebosa” “Endobugula sertula”
Abstract
Larvae of the bryozoan Bugula neritina harbor bacterial symbionts. These symbionts were identified as a novel species of gamma-proteobacterium, based on ribosomal small-subunit rRNA gene sequences. In situ hybridization with oligonucleotides specific for the symbiont confirmed the origin of the sequence. The taxonomic status "Candidatus Endobugula sertula" is proposed for the larval symbiont.

Physiological ecology of Methanobrevibacter cuticularis sp. nov. and Methanobrevibacter curvatus sp. nov., isolated from the hindgut of the termite Reticulitermes flavipes

Citation
Leadbetter, Breznak (1996). Applied and Environmental Microbiology 62 (10)
Names
Methanoflexus curvatus Ts Methanobaculum cuticulare Ts
Abstract
Two morphologically distinct, H2- and CO2-utilizing methanogens were isolated from gut homogenates of the subterranean termite, Reticulitermes-flavipes (Kollar) (Rhinotermitidae). Strain RFM-1 was a short straight rod (0.4 by 1.2 micron), whereas strain RFM-2 was a slightly curved rod (0.34 by 1.6 microns) that possessed polar fibers. Their morphology, gram-positive staining reaction, resistance to cell lysis by chemical agents, and narrow range of utilizable substracts were typical of species b

Dominating Role of an Unusual Magnetotactic Bacterium in the Microaerobic Zone of a Freshwater Sediment

Citation
Spring et al. (1993). Applied and Environmental Microbiology 59 (8)
Names
Magnetobacterium
Abstract
A combination of polymerase chain reaction-assisted rRNA sequence retrieval and fluorescent oligonucleotide probing was used to identify in situ a hitherto unculturable, big, magnetotactic, rod-shaped organism in freshwater sediment samples collected from Lake Chiemsee. Tentatively named “Magnetobacterium bavaricum,” this bacterium is evolutionarily distant from all other phylogenetically characterized magnetotactic bacteria and contains unusually high number