Publications

4866

Abstract The African citrus triozid (ACT), Trioza erytreae (Del Guercio, 1918) (Hemiptera: Triozidae), is a significant pest of citrus in southern Africa serving as a vector of African citrus greening disease associated with the phloem-limited gram-negative bacterium “Candidatus Liberibacter africanus” (CLaf). ACT can also vector another Liberibacter species, “Ca. Liberibacter asiaticus” (CLas) associated with Asian citrus greening. Given the confirmed presence of CLas on the African continent, efforts to monitor populations of ACT as well as associated liberibacters in citrus environments have been enhanced since 2021. Trapping and active collections in citrus surroundings indicated the presence of two triozid species with similar morphology to ACT and five other triozids which were morphologically distinct. This study aimed to characterise the diversity of triozid species around citrus and determine their association with liberibacter. Morphological characters and nucleotide sequence phylogeny of the mitochondrial cytochrome c oxidase I (COI) gene region supported the distinction of seven triozid species including ACT. CLaf was only detected in ACT. None of the other triozid species had any liberibacter species associated with them. Our results suggest the presence of other triozid species in other African countries, which probably were misidentified as ACT. Our findings highlight the need for a comprehensive taxonomic revision of triozids associated with citrus. Integrating morphological and molecular characters in a revised classification will be essential for accurate identification of ACT. Molecular confirmation is needed for confidence in distinguishing ACT from closely related non-pest species.

Abstract Segmented filamentous bacteria (SFB) describe morphologically similar gut commensals found in mammals, fish and birds. In mice, SFB intimately colonizes the ileal epithelium at the time of weaning and elicits a strong pleiotropic immune activation that fosters colonization resistance while augmenting disease severity in various disease models. SFB is therefore critical in both health and disease but information regarding SFB in humans remains limited. Here, we first identify and characterize a human SFB species with SFB-specific morphology, including the hook-like tip structure that mediates attachment, and unique genome features, including a starch and glycogen degradation module. This species, which we name Anisomitus miae and establish as the nomenclature type for the SFB genus, is common across Africa. We then bioinformatically identify, based on the 16S rRNA gene V3-V4 variable region sequence, four major, and two minor, human SFB lineages in forty-four countries distributed across all six inhabited continents. We provide evidence towards the co-colonization potential of the SFB lineages and their colonization dynamics, including a potent but short-lived colonization peak in children between one to five years of age. This study establishes the presence of multiple SFB species in the human population and SFB as a minor but wide-spread group of commensals in humans.

ABSTRACT In this study, a bacterium (NEIST_PMIB_06) was isolated from Hawaijar, a fermented soy food from Manipur, Northeast India. The bacterium was inoculated and incubated to isolate bioactive compounds. Liquid‐liquid extraction was performed to obtain extracts (cell‐free and cell‐based), and their efficacy was evaluated. An extract showing inhibition of α‐amylase and α‐glucosidase was selected for GC‐MS analysis. The anti‐diabetic activity was predicted using Prediction of Activity Spectra for Substances (PASS), and the ADMET properties of the compounds were assessed to identify a suitable compound for further investigation. Molecular docking and molecular dynamic simulation were conducted on twenty‐four compounds, with seven compounds showing potential anti‐diabetic activity based on PASS predictions. In silico analysis revealed the anti‐diabetic activity and ADMET properties of the selected compounds, showing strong affinity with the target receptors. Compounds CIII, CIV, and CVII emerged as promising lead molecules compared to others based on molecular docking results. This study highlights the isolation of a potential bacterium containing diverse anti‐diabetic candidates, supported by in vitro and in silico studies.

ABSTRACT Crape jasmine is a beautiful evergreen flowering shrub and is native to India. During a survey in 2025, severe yellowing symptoms with stunted growth of the plant was observed on the crap jasmine plant at Bhopal, Central India. For the identification of ‘ Candidatus phytoplasma species’ on crap jasmine, symptomatic and asymptomatic leaf samples were collected and total DNA was extracted. The phytoplasma 16S rRNA gene was successfully amplified by direct and nested PCR using isolated DNA and phytoplasma‐specific primers from three symptomatic plants. The amplified ~1.2 kb nested PCR products from three samples were sequenced, and the resulting 16S rRNA gene nucleotide sequences were submitted to GenBank under the accession numbers PX442245, PX890108 and PX890109. Sequence analysis of all three isolates (PX442245, PX890108 and PX890109) revealed the highest nucleotide sequence identity (99%) and close phylogenetic relationships with reference strains of brinjal little leaf phytoplasma, ‘ Candidatus Phytoplasma trifolii’ (16SrVI group), predominantly belonging to the 16SrVI‐D subgroup. In silico RFLP analysis of the 16S rRNA gene sequences of the phytoplasma isolates under study showed the highest similarity to the reference strain of the 16SrVI‐D subgroup, with similarity coefficients ranging from 0.93 to 0.86. To the best of our knowledge, this is the first report of a ‘ Candidatus Phytoplasma trifolii’‐related strain (16SrVI‐D subgroup) associated with yellows disease of crape jasmine ( Tabernaemontana divaricata ) in India.