Genetics

Here, we report the draft genome and annotation of “ Candidatus Nardonella dryophthoridicola” strain NARMHE1, obtained via Oxford Nanopore sequencing of the ovaries of its host, the weevil Metamasius hemipterus , from a population from southeast Brazil.

CLas inhibits callose deposition in the sieve pores and the accumulation of reactive oxygen species to favor its cell-to-cell movement.

AbstractMost prokaryotes are not available as pure cultures and therefore ineligible for naming under the rules and recommendations of the International Code of Nomenclature of Prokaryotes (ICNP). Here we summarize the development of the SeqCode, a code of nomenclature under which genome sequences serve as nomenclatural types. This code enables valid publication of names of prokaryotes based upon isolate genome, metagenome-assembled genome or single-amplified genome sequences. Otherwise, it is similar to the ICNP with regard to the formation of names and rules of priority. It operates through the SeqCode Registry (https://seqco.de/), a registration portal through which names and nomenclatural types are registered, validated and linked to metadata. We describe the two paths currently available within SeqCode to register and validate names, including Candidatus names, and provide examples for both. Recommendations on minimal standards for DNA sequences are provided. Thus, the SeqCode provides a reproducible and objective framework for the nomenclature of all prokaryotes regardless of cultivability and facilitates communication across microbiological disciplines.

Las variedades de papa (Solanum tuberosum L.) producidas en México son susceptibles a Candidatus Liberibacter solanacearum (CaLso), causante de la enfermedad conocida como ‘manchado interno de la pulpa’, por lo que se requiere conocer la respuesta de genotipos experimentales a la bacteria. El presente estudio tuvo como objetivo evaluar el efecto de la infección de los haplotipos LsoA + LsoB de CaLso en el follaje, la biomasa seca y la calidad de tubérculo de papa, variedad Fianna, una colecta de Solanum demissum y los clones experimentales T90-1-63 y T05-13-21 de Solanum spp. El manchado interno de la pulpa del tubérculo se determinó mediante análisis de imágenes de tubérculos. Las plantas de Fianna mostraron la mayor severidad de daño foliar; en cambio, los clones experimentales presentaron 17 % menos daño foliar que Fianna y 8 % más daño foliar que S. demissum. Este último fue el genotipo con la mayor biomasa seca de hoja y produjo tubérculos de un tamaño pequeño; las plantas infectadas de S. demissum presentaron mayor número de tubérculos y mayor rendimiento de tubérculo fresco que las plantas sin inoculación, aunque tuvieron la menor proporción de superficie sana del tubérculo. El clon T90-1-63 presentó los porcentajes más altos de superficie sana de tubérculo (> 79 %) y las menores intensidades del manchado interno de la pulpa del tubérculo.

Methanogens are important hydrogen consumers in the gut and are associated with differing host health. Here, we determine the prevalence and abundance of archaeal species in the guts of a multi-ethnic cohort of healthy Singapore residents.

Here, we report draft genome sequences for nine strains of “ Candidatus Nanosynbacter sp. HMT-352.” These strains and their sequences were used to interrogate strain-level variations in host range, gene content, and growth dynamics among the phylum “ Candidatus Saccharibacteria.”