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First Report of “Candidatus Liberibacter asiaticus”, the Agent of Citrus Huanglongbing (Ex-greening) in Bhutan

Citation
Doe et al. (2003). Plant Disease 87 (4)
Names
Ca. Liberibacter asiaticus
Abstract
Mandarin (Citrus reticulata) is the most important cash crop in Bhutan and plantations total approximately 1.8 million trees (Ministry of Agriculture, Thimphu, Bhutan, 2000). Most trees are a local mandarin variety. Seedlings trees are produced by local farmers or supplied by Druk Seed Nursery. Mandarin seedlings have also been introduced from India. In the mid-1990s, mandarin trees growing in Punakha Valley and Wangdue districts began showing symptoms of decline that included sparse yellow fol

First Report of Citrus Greening Disease and Associated Bacterium “Candidatus Liberibacter asiaticus” from Bhutan

Citation
Ahlawat et al. (2003). Plant Disease 87 (4)
Names
Ca. Liberibacter asiaticus
Abstract
During July 2002, surveys of mandarin orchards were conducted in Punakha Valley and Wangdue districts of Bhutan. Symptoms of the greening disease were observed in most of the orchard. The incidence of disease was recorded up to 30% in 24 private orchards with more than 5,000 trees total. Affected trees were generally stunted with leaves showing symptoms of mottling. Sometimes, symptoms were seen only on one part of the canopy. The greening disease is caused by a fastidious phloem restricted bac

Use of Real-Time PCR To Detect and Quantify Mycoplasma haemofelis and “ Candidatus Mycoplasma haemominutum” DNA

Citation
Tasker et al. (2003). Journal of Clinical Microbiology 41 (1)
Names
Ca. Mycoplasma haemominutum
Abstract
ABSTRACT A real-time PCR assay using Taqman probes was developed to detect and quantify Mycoplasma haemofelis and “ Candidatus Mycoplasma haemominutum” in feline blood samples. The assay was rapid and sensitive and was successfully used to monitor the in vivo kinetics of cats experimentally infected with each species.